Analytes Certified for in 2019
The soil is extracted with boiling 0.01M Cacl2 solution at a 1:2 soil:solution ratio for 10 mins. It is then quickly filtered to avoid any re-fixation of the solubilised B. The extract is then analysed by ICP-AES.
This popular Australian P test on milled air-dry sample is suitable for acidic, neutral and alkaline soils. The extractant is freshly prepared 0.5M sodium bicarbonate @ pH 8.5. The wide soil/extractant ratio of 1:100 and an extended shaking time of 16 h favours readily available and more slowly available forms of soil P, while suppressing the solubility of basic calcium phosphates often found in neutral and alkaline soils. Method 9B1 describes a manual, molybdenum-blue colorimetric procedure with a preferred absorbance at 882 nm, whereas Method 9B2 refers to the same initial soil extraction, followed by an equivalent automated molybdenum-blue colorimetric finish (continuous segmented flow or flow injection analysis). The methods specify reporting results as mg P/kg on an air-dry basis.
This test on milled air-dry sample at a soil/water ratio of 1:5 for 1 h is suitable for use on all soils, irrespective of whether acidic or alkaline. It usually underestimates the soluble salt status of soils containing natural or added gypsum, particularly if ³ 1% of gypsum is present. Such soils would have an EC of about 2 dS/m. Soil EC x 0.336 (Method 3B1) approximates percent total soluble salts, while approximate soil ionic strength (Method 3C1) at 0.1 bar (I0.1) can be calculated as follows: I0.1 = [0.0446*EC1:5 – 0.000173], where I0.1 has units of mM, and EC1:5 has units of dS/m @ 25oC.
Method for measuring exchangeable bases (Ca2+, Mg2+, Na+, K+) - 1M ammonium chloride at pH 7.0
Suited for use on all soils, irrespective of whether acidic or alkaline, but preferred on acidic to weakly alkaline soils not dominated by solid-phase carbonates. Method 15A1 has no pre-treatment to remove soluble salts, with alternatives to remove them chemically (15A2) or to adjust for the presence of soluble sodium (15A3).
Soils are extracted with 0.005M DTPA, at a 1:2 soil:solution ratio, and shaken for 2 hr at 25oC. Analytical finish is either ICP-AES or Flame AAS.
Empirical extraction at 40oC in 0.25M KCl, for 3 hours. Total S in the extract is measured by ICP-AES.
Sulfate sulphur is extracted in the absence of activated charcoal from air-dry soil <2 mm particle size, by 0.01M Ca (H2PO4)2 at pH 4.0 using a soil/solution ratio of 1:5 and an extraction time of 17 h at 25 degrees C. This extracted sulphur is then determined in an aliquot of particle-free soil extract by the method of Johnson and Nishita (1952).
Analysis of Mehlich 3 extract using inductively-coupled atomic emission spectroscopy (ICP-AES)
Initially, milled air-dry soil is extracted for 1 h with 2M KCl at a 1:10 soil/solution ratio. For 7C1a to 7C1h methods, mineral-N components are quantified by steam distillations and subsequent titrations. For 7C2 methods, mineral-N fractions in the clarified soil extract are determined by automated colorimetric procedures. Ammonium ions (NH4+) are measured by a modified Berthelot indophenol reaction, while the Griess-Ilosvay reaction is used for NO3-N (and NO2-N). The methods specify reporting results for NH4-N and NO3-N [plus NO2-N if present], respectively, as mg N/kg on an oven-dry (105oC) basis. Specifically, method 7C2a relates to an automated colour, continuous segmented flow analytical finish, while Method 7C2b codes an automated colour finish by flow injection analysis. Little difference is expected in results due to choice of colorimetric finish.
This measure of soil organic carbon (OCW&B; expressed as %C) usually yields a lower figure than the true total organic carbon value. The method uses finely-milled air-dry sample. It involves wet oxidation by a dichromate-sulphuric acid mixture and relies only on heat of reaction. Soil weight should take account of the expected concentration of OC, and it is expected that allowance will be made for positive soluble Clâ€‘ interference in soils containing >0.5% Cl. The method specifies reporting on an oven-dry (105oC) basis. Nowadays this method is less preferred than 6B methods.
This index of soil P sorption embraces adsorption as well as precipitation reactions. Equilibrated soil extracts are obtained by shaking milled air-dry soil continuously for 17 h at a ratio of 1:10 (w/v) with a P equilibrating solution initially containing the equivalent of 1000 mg P/kg in 0.01M CaCl2. The residual P in the extract is determined using the Murphy and Riley colorimetric finish. In other related methods (9I2a, 9I3a) the PBI value is modified to allow for the soil fertility level. In this method, there is nosuch adjustment. The methods specify reporting results on an air-dry basis.
The laboratory either did not specify the method used or uses a mix of methods when submitting to the certification body
Tests for water-soluble chloride (Cl) on milled air-dry sample are suitable for use on all soils. For method 5A1, Clâ€‘ in clarified 1:5 soil/water extracts is determined by potentiometric titration with AgNO3 in conjunction with an Ag/AgNO3 electrode array. For method 5A2a, Clâ€‘ in clarified 1:5 soil/water extracts is determined by an automated, continuous flow colorimetric procedure based on the formation — in the presence of ferric ions and free thiocyanate ions — of highly coloured ferric thiocyanate in proportion to the Clâ€‘ concentration. Method 5A2b is similar, except it pertains to the use of flow injection analysis (FIA). For 5A1 and 5A2 methods, it is assumed there are no chemical interferences of significance. Moreover, Method 5A2a has proven more precise than method 5A1, particularly at soil concentrations <50 mg Cl/kg. Other analytical finish options involve chemically-suppressed ion chromatography (5A3a), single-column electronically suppressed ion chromatography (5A3b), and direct measurement by ICPAES (Method 5A4). The methodology specifies reporting results on an air-dry basis.
Microwave Digestion - closed system without HF, and final medium HNO3 and/or HCl. ICP-AES finish.
Extraction with water. Spectrophotometric (automated) finish.
Finely divided dry sample. Dumas combustion.