Analytes Certified for in 2020
Initially, milled air-dry soil is extracted for 1 h with 2M KCl at a 1:10 soil/solution ratio. For 7C1a to 7C1h methods, mineral-N components are quantified by steam distillations and subsequent titrations. For 7C2 methods, mineral-N fractions in the clarified soil extract are determined by automated colorimetric procedures. Ammonium ions (NH4+) are measured by a modified Berthelot indophenol reaction, while the Griess-Ilosvay reaction is used for NO3-N (and NO2-N). The methods specify reporting results for NH4-N and NO3-N [plus NO2-N if present], respectively, as mg N/kg on an oven-dry (105oC) basis. Specifically, method 7C2a relates to an automated colour, continuous segmented flow analytical finish, while Method 7C2b codes an automated colour finish by flow injection analysis. Little difference is expected in results due to choice of colorimetric finish.
Analysis of Mehlich 3 extract using inductively-coupled atomic emission spectroscopy (ICP-AES)
This method for total soil OC involves production, purification and measurement of CO2 evolved when soil carbon is ignited in a stream of O2. Because all C compounds are converted to CO2, the C from carbonates, charcoal, undecomposed wood, etc, will be included, as no soil pre-treatment is specified. In the volumetric sub-method 6B2a, concentrated KOH solution is used to absorb the CO2 released. The difference between the original volume of gas in the burette and the volume produced after ignition equals the volume of CO2 evolved from the sample, after correction for gas temperature and pressure. Sub-method 6B2b is similar to Method 6B2a, except the CO2 produced by ignition is measured via infrared / thermal conductivity detection. Both 6B2a and 6B2b use finely-milled air-dry sample, with weights varying with expected C concentrations. Surrogate estimates can be obtained by NIR (method 6B4a) or MIR (method 6B4b) reflectance spectroscopy. The methods specify reporting as %C on an oven-dry (105oC) basis.
Microwave Digestion - closed system without HF, and final medium HNO3 and/or HCl. ICP-AES finish.
Microwave Digestion - closed system without HF, and final medium HNO3 and/or HCl. ICP-MS finish.
Finely divided dry sample. Dumas combustion.
Extraction with acid(s). Titrimetric finish.
Extraction with acid(s). Spectrophotmetry (automated) finish.